https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 Catalytic efficiency is a better predictor of arsenic toxicity to soil alkaline phosphatase https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32983 max) and Michaelis constant (Km) in unpolluted soils were 0.012-0.267 mM h-1 and 1.34-3.79 mM respectively. The competitive inhibition constant (Kic) was 0.17-0.70 mM, which was lower than Km, suggesting higher enzyme affinity for As than for substrate. The ecological doses, ED₁₀ and ED₅₀ (concentration of As that results in 10% and 50% inhibition on enzyme parameter) for inhibition of catalytic efficiency (Vmax/Km) were lower than those for inhibition of enzyme activity at different substrate concentrations. This suggests that the integrated kinetic parameter, catalytic efficiency is substrate concentration independent and more sensitive to As than ALP activity. Thus, catalytic efficiency was proposed as a more reliable indicator than ALP activity for risk assessment of As pollution.]]> Fri 17 Aug 2018 15:44:20 AEST ]]> Arsenate inhibition on kinetic characteristics of alkaline phosphatase as influenced by pH https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43401 Fri 16 Sep 2022 09:58:33 AEST ]]>